2P44
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 1.8A resolution: SE5A-mono-1 crystal form with five se-met sites (M34, M51, F68M, M83, L86M) in vhh scaffold
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-01 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97955, 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.783, 54.765, 49.064 |
| Unit cell angles | 90.00, 109.57, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.19544 |
| Rwork | 0.193 |
| R-free | 0.23820 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.410 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.095 |
| Number of reflections | 18982 |
| Completeness [%] | 99.9 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 295 | HAMPTON RESEARCH INDEX SCREEN, SOLUTION #85: MGCL2, PEG 3350, TRIS BUFFER, PH 8.5, VAPOR DIFFUSION, temperature 295K |
