2P3Y
Crystal structure of VPA0735 from Vibrio parahaemolyticus. NorthEast Structural Genomics target VpR109
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.764, 64.253, 78.524 |
| Unit cell angles | 84.81, 89.14, 76.47 |
Refinement procedure
| Resolution | 35.700 - 1.800 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.027 | 0.121 |
| Number of reflections | 194335 | |
| <I/σ(I)> | 27.5 | 24.6 |
| Completeness [%] | 89.4 | 95.6 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 200mM MOPS, PEG8000, MgCl2, 10mM DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
