2P3P
Structure of a domain of an uncharacterized protein PG_1388 from Porphyromonas gingivalis W83
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924, 0.97938 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.753, 48.259, 54.191 |
| Unit cell angles | 86.55, 79.58, 68.35 |
Refinement procedure
| Resolution | 35.000 - 1.760 |
| R-factor | 0.16849 |
| Rwork | 0.165 |
| R-free | 0.22645 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.566 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.070 | 0.190 |
| Number of reflections | 37374 | |
| <I/σ(I)> | 14.4 | 6 |
| Completeness [%] | 92.9 | 71.7 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2M Ammonium acetate, 0.1M BIS-TRIS pH 6.5, 25 % w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
