2P3J
Crystal structure of the Arg101Ala mutant protein of Rhesus rotavirus VP8*
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2004-12-21 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 48.531, 48.531, 131.403 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.640 - 1.900 |
| R-factor | 0.1629 |
| Rwork | 0.161 |
| R-free | 0.20586 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | RRV VP8* PDB code 1KQR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.217 |
| Data reduction software | SAINT |
| Data scaling software | LSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.040 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.107 |
| Number of reflections | 12064 | |
| <I/σ(I)> | 11.8 | 4.7 |
| Completeness [%] | 91.7 | 63 |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 303 | 1.7M (NH4)2SO4, 2.4% v/v PEG 400, 0.1M PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
