2P3I

Crystal structure of Rhesus Rotavirus VP8* at 295K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	295
Detector technology	CCD
Collection date	2004-07-01
Detector	BRUKER SMART 6000
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	48.549, 48.549, 131.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.750
R-factor	0.16388
Rwork	0.163
R-free	0.18773
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	RRV VP8* structure PDB code 1KQR
RMSD bond length	0.009
RMSD bond angle	1.304
Data reduction software	SAINT
Data scaling software	LSCALE
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.540	1.830
High resolution limit [Å]	1.750	1.750
Rmerge	0.063	0.300
Number of reflections	16731
<I/σ(I)>	8.8	1.9
Completeness [%]	99.7	100
Redundancy	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	303	1.7M (NH4)2SO4, 2.4% v/v PEG 400, 0.1M PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K