2P32

Crystal structure of the C-terminal 10 kDa subdomain from C. elegans Hsp70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-05-04
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.978
Spacegroup name	P 42 21 2
Unit cell lengths	138.927, 138.927, 100.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.000 - 3.200
Rwork	0.268
R-free	0.28190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Preliminary model built using data from a mercury derivative crystal solved using MAD
RMSD bond length	0.018
RMSD bond angle	1.761
Data reduction software	MOSFLM
Data scaling software	SCALA (CCP4_3.2.17)
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.136	0.936
Total number of observations		11782
Number of reflections	16809
<I/σ(I)>	12.9	2
Completeness [%]	99.9	100
Redundancy	8.7	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	55% ammonium sulphate, 0.5% PEG 400, 0.1M sodium citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K