2P2V
Crystal structure analysis of monofunctional alpha-2,3-sialyltransferase Cst-I from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-16 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | I 4 |
| Unit cell lengths | 112.388, 112.388, 58.757 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.750 - 1.850 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1RO7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.994 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.064 | 0.050 | 0.524 |
| Number of reflections | 40593 | ||
| <I/σ(I)> | 21.4 | 2.6 | |
| Completeness [%] | 99.8 | 99.6 | 99 |
| Redundancy | 10.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20mM Tris-HCl pH 7.5, and 200mM NaCl |
