2P2U
Crystal structure of putative host-nuclease inhibitor protein Gam from Desulfovibrio vulgaris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.030, 93.408, 104.166 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.750 |
| R-factor | 0.245 |
| Rwork | 0.242 |
| R-free | 0.29000 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.197 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.976 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.143 | 0.475 |
| Number of reflections | 15027 | |
| <I/σ(I)> | 12.3 | 3.3 |
| Completeness [%] | 98.5 | 98.2 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 294 | 100mM Tris-HCl pH 9.0, 21% PEG 4000, 200mM Sodium acetate, VAPOR DIFFUSION, temperature 294K |
