2P2N
Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.01259 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.315, 89.757, 93.083 |
| Unit cell angles | 90.00, 117.03, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.223 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.369 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 95017 | |
| <I/σ(I)> | 17.9 | 5 |
| Completeness [%] | 90.6 | |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 291 | citric acid, sodium chloride, pH 4.0, VAPOR DIFFUSION, temperature 291.0K |
