2P2K
Crystal structure of a lectin from Canavalia gladiata seeds (CGL) in complex with man1-4man-OMe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-01 |
| Detector | MAR CCD 130 mm |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.932, 68.932, 160.436 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.840 - 1.980 |
| R-factor | 0.18453 |
| Rwork | 0.182 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.128 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.096 | |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.068 | 0.233 |
| Number of reflections | 56114 | |
| <I/σ(I)> | 8.8 | 3.2 |
| Redundancy | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4.5-6.5 M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
