2P2D
Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-06 |
Detector | MARRESEARCH |
Wavelength(s) | 1.01259 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.763, 151.586, 87.142 |
Unit cell angles | 90.00, 88.63, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.890 |
Rwork | 0.184 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wls |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.880 | 1.880 |
Number of reflections | 116888 | |
<I/σ(I)> | 18.2 | 4.4 |
Completeness [%] | 96.2 | 85.8 |
Redundancy | 4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1M MES, 22.5% MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |