2P25
The crystal structure of the glyoxalase family protein from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.695, 67.893, 87.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.770 - 1.700 |
| R-factor | 0.20212 |
| Rwork | 0.201 |
| R-free | 0.22958 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.484 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.770 | 1.742 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.077 | 0.355 |
| Number of reflections | 15655 | |
| <I/σ(I)> | 26.92 | 3 |
| Completeness [%] | 96.9 | 95.18 |
| Redundancy | 8.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 1.1M di-Ammonium Tartrate, 0.1M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
