2P1Z
Crystal structure of phosphoribosyltransferase from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929, 0.97943 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 72.438, 103.709, 39.417 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.410 - 2.440 |
| R-factor | 0.2297 |
| Rwork | 0.226 |
| R-free | 0.30847 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.389 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.082 | 0.522 |
| Number of reflections | 12900 | |
| <I/σ(I)> | 32.53 | 1.76 |
| Completeness [%] | 87.5 | 40.9 |
| Redundancy | 7.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Magnesium chloride, 0.1 M Tris-HCl, 25 % PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
