2P1Y
1.B2.D9, a bispecific alpha/beta TCR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.681, 105.477, 81.531 |
| Unit cell angles | 90.00, 116.04, 90.00 |
Refinement procedure
| Resolution | 40.390 - 2.420 |
| R-factor | 0.18508 |
| Rwork | 0.181 |
| R-free | 0.25363 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u3h |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.529 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.400 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.041 | 0.196 |
| Number of reflections | 41336 | |
| <I/σ(I)> | 24 | 6.29 |
| Completeness [%] | 99.1 | 96.4 |
| Redundancy | 4.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | 12% w/w PEG 6000, 100 mM HEPES, 3% v/v MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
