2P1X
Crystal structure analysis of the complex between CyaY and Eu(III)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 43.859, 43.859, 98.717 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.420 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ew4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.086 | 0.419 |
| Number of reflections | 36600 | |
| <I/σ(I)> | 15.9 | 3.1 |
| Completeness [%] | 97.2 | 99.9 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | Crystallization conditions: protein concentration 15mg/ml, 0.1M sodium acetate, 0.2M CaCl2, 30% PEG 4000, 2mM beta mercaptoethanol Soaking conditions: EuCl3 in the stabilizing solution, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
