2P1S
Crystal structure of the C-terminal lobe of bovine lactoferrin complexed with O-alpha-D-Glucopyranosyl-(1 3)-alpha-D-fructofuranosyl- (2 1)- alpha-D-glucopyranoside at 1.93 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54132 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.155, 50.425, 65.883 |
| Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
| Resolution | 63.250 - 1.930 |
| R-factor | 0.17441 |
| Rwork | 0.170 |
| R-free | 0.21652 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o1l |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.689 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.250 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 26745 | |
| Completeness [%] | 94.1 | 83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.1M HEPES, 0.2M ZnSO4, 20% PEG monomethyl ether, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
