2P1O
Mechanism of Auxin Perception by the TIR1 ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.971, 82.456, 125.320 |
| Unit cell angles | 90.00, 103.84, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.18618 |
| Rwork | 0.184 |
| R-free | 0.23097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p2m |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.641 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Number of reflections | 70515 | |
| <I/σ(I)> | 27.2 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM BTP, 10% 14% PEG 20,000, 200 mM NaCl, 5 mM DTT, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
