2P1J
Crystal structure of a polC-type DNA polymerase III exonuclease domain from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.703, 124.939, 143.062 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.232 |
| Rwork | 0.229 |
| R-free | 0.29800 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.958 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.050 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.106 | 0.816 |
| Number of reflections | 17030 | |
| <I/σ(I)> | 14.9 | 1.5 |
| Completeness [%] | 96.4 | 91.5 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.6 | 294 | 100mM Tris-HCl pH 8.6, 20% Glycerol, 14% PEG 10000, 150mM Sodium chloride, VAPOR DIFFUSION, temperature 294K |
