2P1J
Crystal structure of a polC-type DNA polymerase III exonuclease domain from Thermotoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-24 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 55.703, 124.939, 143.062 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.232 |
Rwork | 0.229 |
R-free | 0.29800 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.958 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.050 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.106 | 0.816 |
Number of reflections | 17030 | |
<I/σ(I)> | 14.9 | 1.5 |
Completeness [%] | 96.4 | 91.5 |
Redundancy | 7.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.6 | 294 | 100mM Tris-HCl pH 8.6, 20% Glycerol, 14% PEG 10000, 150mM Sodium chloride, VAPOR DIFFUSION, temperature 294K |