2P1H

Rapid Folding and Unfolding of Apaf-1 CARD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	33.468, 47.515, 54.746
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.900 - 1.590
Rwork	0.239
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cy5
RMSD bond length	0.006
RMSD bond angle	1.145
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	35.900
High resolution limit [Å]	1.590
Number of reflections	5981
Completeness [%]	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	291	200 mM Tris-HCl, pH 6.1, 200 mM zinc acetate, 17% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 291K