2P1F
Human UMP Synthase (C-terminal Domain-Orotidine 5'-Monophosphate Decarboxylase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90020 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.314, 116.875, 61.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.090 - 1.760 |
| R-factor | 0.14113 |
| Rwork | 0.139 |
| R-free | 0.17067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eaw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.503 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.090 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.097 | 0.459 |
| Number of reflections | 26969 | |
| <I/σ(I)> | 9 | 4.49 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 2.28M Ammonium sulfate, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
