2P0S
Structural Genomics, the crystal structure of a putative ABC transporter domain from Porphyromonas gingivalis W83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.880, 59.965, 110.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.560 - 1.600 |
| R-factor | 0.17936 |
| Rwork | 0.177 |
| R-free | 0.22475 |
| Structure solution method | SAD |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.566 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.560 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.085 | 0.478 |
| Number of reflections | 31663 | |
| <I/σ(I)> | 43.87 | 2.35 |
| Completeness [%] | 96.0 | 66.1 |
| Redundancy | 9.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 0.1M MgCl2, 0.1M NaCl, 30% PEG400, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
