2P0N
NMB1532 protein from Neisseria meningitidis, unknown function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.579, 59.558, 59.706 |
| Unit cell angles | 90.00, 110.32, 90.00 |
Refinement procedure
| Resolution | 31.650 - 1.410 |
| R-factor | 0.1696 |
| Rwork | 0.168 |
| R-free | 0.20310 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.642 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.650 | 1.440 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.087 | 0.475 |
| Number of reflections | 62114 | |
| <I/σ(I)> | 25.9 | 1.92 |
| Completeness [%] | 94.6 | 61.1 |
| Redundancy | 6.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2 Magnesium chloride, 0.1 M Bis-Tris buffer, 25% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
