2P0L
Crystal structure of a Lipoate-protein ligase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.961, 49.245, 61.452 |
| Unit cell angles | 90.00, 99.22, 90.00 |
Refinement procedure
| Resolution | 40.060 - 2.040 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.096 | 0.414 |
| Number of reflections | 17861 | |
| <I/σ(I)> | 11.1 | 1 |
| Completeness [%] | 99.5 | 96.1 |
| Redundancy | 4.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1M Tris-HCl pH 8.5, 30% PEG4000, 0.2M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
