2P0K
Crystal structure of SCMH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 95.735, 95.735, 43.602 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.050 - 1.750 |
| R-factor | 0.17065 |
| Rwork | 0.169 |
| R-free | 0.20351 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2biv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.396 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 83.050 |
| High resolution limit [Å] | 1.750 |
| Rmerge | 0.052 |
| Number of reflections | 23100 |
| <I/σ(I)> | 13.5 |
| Completeness [%] | 99.3 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 300 | 20% PEG3350, 0.2M Ammonium Citrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
