2P0I
Crystal structure of L-rhamnonate dehydratase from Gibberella zeae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 201.404, 201.135, 82.402 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.19197 |
| Rwork | 0.190 |
| R-free | 0.25589 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0028) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.085 | 0.360 |
| Number of reflections | 187072 | |
| <I/σ(I)> | 5.2 | 1 |
| Completeness [%] | 95.6 | 92.7 |
| Redundancy | 4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 0.1M Tris-HCl, pH 7.0, 2M Ammonium sulfate, 0.2M Lithium sulfate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
