2P0H
ArhGAP9 PH domain in complex with Ins(1,3,4)P3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.12714 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.920, 36.550, 112.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.010 - 1.900 |
| R-factor | 0.19677 |
| Rwork | 0.194 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p0f |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.989 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.044 | 0.225 |
| Number of reflections | 9921 | |
| <I/σ(I)> | 12.6 | 3.33 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% PEG4000, 100 mM HEPES, 5% glycerol, 5mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
