2P0A
The crystal structure of human synapsin III (SYN3) in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99991 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.796, 73.186, 78.398 |
| Unit cell angles | 90.00, 100.74, 90.00 |
Refinement procedure
| Resolution | 47.000 - 1.900 |
| R-factor | 0.18311 |
| Rwork | 0.181 |
| R-free | 0.21804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Swissmodel based on PDB entries: 1I7L 1i7n 1pk8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.358 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.280 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.132 | 0.477 |
| Number of reflections | 56940 | |
| Completeness [%] | 99.2 | 94.4 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.26M (NH4)2SO4, 0.2M Li2SO4, Tris-HCl pH 8.5, 20 % Ethylene glycol added as cryoprotectant, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
