2P06
Crystal structure of a predicted coding region AF_0060 from Archaeoglobus fulgidus DSM 4304
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920, 0.97940 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.083, 80.083, 106.771 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.670 - 2.100 |
| R-factor | 0.17006 |
| Rwork | 0.169 |
| R-free | 0.18655 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.329 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.400 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.121 | 0.790 |
| Number of reflections | 23576 | |
| <I/σ(I)> | 30.6 | 4.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 14.6 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 291 | 0.2M Potassium thiocyanate, 30% PEG 3350, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
