2P04
2.1 Ang structure of the dimerized PAS domain of signal transduction histidine kinase from Nostoc punctiforme PCC 73102 with homology to the H-NOXA/H-NOBA domain of the soluble guanylyl cyclase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.210, 44.362, 59.740 |
| Unit cell angles | 90.00, 104.51, 90.00 |
Refinement procedure
| Resolution | 25.260 - 2.110 |
| R-factor | 0.20232 |
| Rwork | 0.199 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Truncated delta-7 H-NOXA structure |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.349 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 12364 | |
| <I/σ(I)> | 10 | 1.6 |
| Completeness [%] | 87.4 | 59.8 |
| Redundancy | 3.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Hepes pH 7.5, 1.5M LiSO4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
