2OZ1
The SoxAX Complex of Rhodovulum Sulfidophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.975, 102.949, 115.706 |
Unit cell angles | 90.00, 110.22, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.350 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h33 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.331 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 108.577 | 54.310 | 2.470 |
High resolution limit [Å] | 2.345 | 7.410 | 2.340 |
Rmerge | 0.086 | 0.065 | 0.189 |
Total number of observations | 6279 | 13326 | |
Number of reflections | 71803 | ||
<I/σ(I)> | 7.4 | 7.5 | 3.7 |
Completeness [%] | 96.0 | 95.4 | 81.5 |
Redundancy | 2.7 | 2.7 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 15% (w/v) PEG 4K, 0.2 M magnesium acetate, 100 mM sodium MES buffer pH 6.5. The crystals could be cryoprotected by transferring them to stabilization solution containing 25% (v/v) ethylene glycol. , VAPOR DIFFUSION, HANGING DROP, temperature 277K |