2OYZ
Crystal structure of unknown function protein VPA0057 from Vibrio parahaemolyticus (targeted domain 2-94)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 37.302, 37.302, 135.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.290 - 1.710 |
| R-factor | 0.20461 |
| Rwork | 0.203 |
| R-free | 0.23657 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.770 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.075 | 0.352 |
| Number of reflections | 11064 | |
| <I/σ(I)> | 72 | 4.52 |
| Completeness [%] | 99.1 | 93.5 |
| Redundancy | 13.4 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 0.1M CaCl2, 0.1M Sodium acetate pH 4.6, 15% PEG 400, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
