2OYP
T Cell Immunoglobulin Mucin-3 Crystal Structure Revealed a Galectin-9-independent Binding Surface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.4503 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.000, 52.809, 38.941 |
| Unit cell angles | 90.00, 100.94, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.952 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eaj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.195 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.073 | 0.136 |
| Number of reflections | 6457 | |
| <I/σ(I)> | 12 | 6.8 |
| Completeness [%] | 96.2 | 72.6 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 1.8 M Ammonium Sulfate, 0.1 M Sodium Citrate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
