2OYO

Crystal structure of Uncharacterized peroxidase-related protein (YP_604910.1) from Deinococcus geothermalis DSM 11300 at 1.51 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97916, 0.97886
Spacegroup name	H 3 2
Unit cell lengths	135.649, 135.649, 118.084
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.163 - 1.510
R-factor	0.184
Rwork	0.182
R-free	0.21500
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.497
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.163		1.560
High resolution limit [Å]	1.510	2.580	1.510
Rmerge	0.138	0.081	0.666
Number of reflections	64521
<I/σ(I)>	9.57	18.7	1.8
Completeness [%]	96.5	99.7	84.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	NANODROP, 40.0% MPD, 0.1M Bicine pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K