2OYN
Crystal structure of CDP-bound protein MJ0056 from Methanococcus jannaschii, Pfam DUF120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.480, 63.480, 73.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23900 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.646 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.946 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.137 | 0.866 |
| Number of reflections | 13339 | |
| <I/σ(I)> | 26.6 | 4.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 27.9 | 28.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 100mM Hepes pH 7.5, 10% Isopropanol, 17% PEG 4000, VAPOR DIFFUSION, temperature 294K |
