2OY9
Crystal structure of an unknown conserved protein- Pfam: UPF0223
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 65 |
| Unit cell lengths | 91.568, 91.568, 47.241 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.780 - 1.600 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.086 | 0.610 |
| Number of reflections | 28045 | |
| <I/σ(I)> | 13.8 | 1 |
| Completeness [%] | 93.5 | 62 |
| Redundancy | 19.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Bis-Tris pH 6.5, 25% PEG3350, 0.2M MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
