2OY1
The crystal structure of OspA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.046, 56.005, 66.204 |
Unit cell angles | 90.00, 97.09, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.860 |
R-factor | 0.20764 |
Rwork | 0.206 |
R-free | 0.23635 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8c |
RMSD bond length | 0.023 |
RMSD bond angle | 1.971 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.084 | 0.084 |
Number of reflections | 22098 | |
<I/σ(I)> | 13.9 | 3.61 |
Completeness [%] | 99.8 | 100 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 26% PEG3350, 0.1M Imidazole, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |