2OXN
Vibrio cholerae family 3 glycoside hydrolase (NagZ) in complex with PUGNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.734, 67.850, 96.894 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.560 - 1.700 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tr9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.298 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.560 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.061 | 0.167 |
| Number of reflections | 35787 | |
| <I/σ(I)> | 27.2 | 8.4 |
| Completeness [%] | 99.3 | 95.5 |
| Redundancy | 7.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 15% PEG 20000, 100mM Bis-Tris, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
