2OXL

Structure and Function of the E. coli Protein YmgB: a Protein Critical for Biofilm Formation and Acid Resistance

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-25
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9790, 0.9793, 0.9322
Spacegroup name	C 2 2 21
Unit cell lengths	69.919, 69.945, 55.021
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.21567
Rwork	0.215
R-free	0.23789
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.666
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.11)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.038	0.403
Number of reflections	12802
<I/σ(I)>	22.8
Completeness [%]	99.6	96.8
Redundancy	6.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8	295	90 mM Tris, 1.8 M NaCl, 0.5 (w/v)% B-OG, pH 8.0, microbatch, temperature 295K
1	MICROBATCH	8	295	90 mM Tris, 1.8 M NaCl, 0.5 (w/v)% B-OG, pH 8.0, microbatch, temperature 295K
1	MICROBATCH	8	295	90 mM Tris, 1.8 M NaCl, 0.5 (w/v)% B-OG, pH 8.0, microbatch, temperature 295K