2OXH
The SOXYZ Complex of Paracoccus Pantotrophus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.220, 54.490, 104.872 |
| Unit cell angles | 103.13, 95.21, 90.01 |
Refinement procedure
| Resolution | 50.000 - 2.350 |
| R-factor | 0.19659 |
| Rwork | 0.194 |
| R-free | 0.24433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ox5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.376 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.687 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.105 | 0.144 |
| Number of reflections | 34657 | |
| <I/σ(I)> | 4.1 | 4.1 |
| Completeness [%] | 96.4 | 93.3 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1 microL of 10 mg ml-1 protein in 10 mM Tris pH 8.0 with 1 microL of 100 mM Na-acetate pH 4.75, 200 mM (NH4)2SO4, 31 % (w/v) PEG 3350, 2% (w/v) glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
