2OXF

X-ray structure of the unliganded uridine phosphorylase from Salmonella typhimurium in homodimeric form at 1.76A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Detector	MAR CCD 165 mm
Spacegroup name	H 3
Unit cell lengths	150.860, 150.860, 47.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.760
Rwork	0.163
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.752
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	150.860
High resolution limit [Å]	1.760
Number of reflections	39165

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	VAPOR DIFFUSION, HANGING DROP, temperature 297K