2OX9
Mouse Scavenger Receptor C-type Lectin carbohydrate-recognition domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.998, 53.755, 59.084 |
| Unit cell angles | 67.75, 76.70, 85.37 |
Refinement procedure
| Resolution | 53.390 - 1.950 |
| Rwork | 0.224 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Phasing software | COMO |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.700 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 35627 | |
| Completeness [%] | 92.2 | 91.7 |
| Redundancy | 2.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | The protein solution contained 6mg ml-1 protein, 9mM CaCl2 , 9mM Tris pH=7.8, 22.5mM NaCl and 5mM Lewisx. The reservoir solution contained 30% Peg 8K, 0.2M NaCl and 0.1 M Imidazole, pH 8.5., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
