2OWZ
R-state, citrate and Fru-6-P-bound Escherichia coli fructose-1,6-bisphosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-11 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 45.315, 83.332, 173.415 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.840 - 2.180 |
| R-factor | 0.212 |
| Rwork | 0.208 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gq1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.840 | 2.320 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.033 | 0.205 |
| Total number of observations | 643 | |
| Number of reflections | 17445 | |
| <I/σ(I)> | 22.7 | 6.5 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 295 | 50 mM sodium citrate pH 5.3, 20% (w/v) PEG 3350, and 20% (w/v) sucrose, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
