2OWY

The recombination-associated protein RdgC adopts a novel toroidal architecture for DNA binding

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-23
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000, 0.95000, 0.97913, 0.97897
Spacegroup name	P 21 21 2
Unit cell lengths	87.494, 116.041, 76.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
Rwork	0.234
R-free	0.27900
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.03)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.054	0.279
Number of reflections	27126
<I/σ(I)>	21.9
Completeness [%]	100.0	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	0.1M sodium HEPES (pH 7.5), 1.0M tri-sodium citrate, 1%(w/v) Anapoe 35, VAPOR DIFFUSION, HANGING DROP, temperature 297K
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	0.1M sodium HEPES (pH 7.5), 1.0M tri-sodium citrate, 1%(w/v) Anapoe 35, VAPOR DIFFUSION, HANGING DROP, temperature 297K
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	0.1M sodium HEPES (pH 7.5), 1.0M tri-sodium citrate, 1%(w/v) Anapoe 35, VAPOR DIFFUSION, HANGING DROP, temperature 297K
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	0.1M sodium HEPES (pH 7.5), 1.0M tri-sodium citrate, 1%(w/v) Anapoe 35, VAPOR DIFFUSION, HANGING DROP, temperature 297K