2OWL

Crystal structure of E. coli RdgC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	180
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	51.425, 95.683, 171.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.800 - 2.400
R-factor	0.24107
Rwork	0.232
R-free	0.32641
Structure solution method	SAD
RMSD bond length	0.033
RMSD bond angle	2.772
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.800
High resolution limit [Å]	2.400
Rmerge	0.060
Number of reflections	29976
Completeness [%]	98.5
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	283	10% (w/v) PEG1000, 0.2 M Ca chloride, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K