2OW4
Crystal structure of a lectin from Canavalia maritima seeds (ConM) in complex with man1-2man-OMe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-01 |
Detector | MAR CCD 130 mm |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 64.252, 89.094, 85.719 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.820 - 1.600 |
R-factor | 0.20769 |
Rwork | 0.206 |
R-free | 0.23039 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.584 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.820 | 1.641 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.055 | 0.452 |
Number of reflections | 30905 | |
<I/σ(I)> | 15.5 | 2.4 |
Completeness [%] | 25.0 | |
Redundancy | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |