2OVV

Crystal structure of the catalytic domain of rat phosphodiesterase 10A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS V
Spacegroup name	H 3
Unit cell lengths	120.657, 120.657, 83.273
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.550 - 2.000
R-factor	0.177
Rwork	0.174
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.534
Data scaling software	d*TREK (8.0SSI)
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.120	65.120	1.740
High resolution limit [Å]	1.680	3.620	1.680
Rmerge	0.093	0.057	0.311
Total number of observations		29445	1447
Number of reflections	42604
<I/σ(I)>	10.1	22.5	2.5
Completeness [%]	83.0	99.3	15.8
Redundancy	4.73	5.61	1.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1