2OVU
Crystal structure of a lectin from Canavalia gladiata (CGL) in complex with man1-2man-OMe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-01 |
| Detector | MAR CCD 130 mm |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 63.887, 86.198, 88.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.770 - 1.500 |
| R-factor | 0.21381 |
| Rwork | 0.213 |
| R-free | 0.22998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.540 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.030 | 1.539 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.061 | 0.061 |
| Number of reflections | 39568 | |
| <I/σ(I)> | 7.6 | 7.6 |
| Completeness [%] | 5.2 | |
| Redundancy | 19.2 | 19.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
