2OVM
Progesterone Receptor with Bound Asoprisnil and a Peptide from the Co-Repressor NCoR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-24 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.514, 87.514, 90.412 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PR LBD from 1A28 minus residues 930-933 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.201 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.100 |
| Number of reflections | 12366 |
| <I/σ(I)> | 25.5 |
| Completeness [%] | 97.0 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | 0.1M Tris, 0.15M NaCl, 10% glycerol, pH 8.0, spontaneous crystallization, temperature 277K |
