2OVL
Crystal structure of a racemase from Streptomyces coelicolor A3(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 177.603, 177.603, 111.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.050 - 2.130 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.228 | |
| Number of reflections | 100173 | |
| <I/σ(I)> | 12.1 | 2.5 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 28.6 | 27.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 3M Sodium acetate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
