2OVG
Lambda Cro Q27P/A29S/K32Q triple mutant at 1.35 A in space group P3221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.524, 50.524, 48.148 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.770 - 1.350 |
| R-factor | 0.134 |
| Rwork | 0.132 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6CRO protein portion |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.067 | 0.540 |
| Number of reflections | 15657 | |
| <I/σ(I)> | 14.5 | 2.96 |
| Completeness [%] | 97.7 | 96.5 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.5 M lithium sulfate, 0.1 M Na Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
